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Part:BBa_K1598007:Design

Designed by: Yash Mishra   Group: iGEM15_UCL   (2015-09-18)


J23101 promoter+RBS+FDXR+RBS+FDX1+ RBS + CYP11A1 gene+rrnb double terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 25
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 25
    Illegal NheI site found at 3264
    Illegal NotI site found at 31
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 25
    Illegal XhoI site found at 554
    Illegal XhoI site found at 1446
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 25
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 25
    Illegal XbaI site found at 40
    Illegal NgoMIV site found at 1642
    Illegal AgeI site found at 1079
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to make sure that that the genes for Cytochrome P450scc and all it's electron transport proteins were included in the biobrick and came after ribosome binding sites. The biobrick was broken into 5 parts for quick synthesize and assembled using Golden Gate assembly. Hence, each part was made compatible for golden gate assembly, and had overhangs for SapI.


Source

http://www.ncbi.nlm.nih.gov/nuccore/153218645?report=fasta http://www.ncbi.nlm.nih.gov/nuccore/16877631?report=fasta http://www.ncbi.nlm.nih.gov/nuccore/39645178?report=fasta https://parts.igem.org/cgi/partsdb/puttext.cgi https://parts.igem.org/cgi/partsdb/puttext.cgi

References